Isolation of salt-soluble elastin from ligamentum nuchae of copper-deficient calf.
نویسندگان
چکیده
This paper describes the isolation and amino acid analysis of un-cross-linked elastin obtained by neutral salt extraction from the ligamentum nuchae of a calf fed from birth to 9 months on a diet low in copper.
منابع مشابه
Carbohydrate content of insoluble elastins prepared from adult bovine and calf ligamentum nuchae and tropoelastin isolated from copper-deficient porcine aorta.
1. Insoluble elastin has been prepared by several different methods from adult bovine and calf ligamentum nuchae. Highly purified tropoelastin has been prepared from copper-deficient porcine aorta. 2. Amino acid analyses indicated that all preparations, except that obtained from calf ligamentum nuchae by using an EDTA extraction followed by collagenase digestion (preparation E6), were typical o...
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Fetal bovine ligamentum nuchae fibroblasts maintained in culture synthesized soluble elastin but were unable to form the insoluble elastic fiber. Secreted elastin precursors accumulated in culture medium and were measured using a radioimmunoassay for elastin. When elastin production was examined in ligament tissue from fetal calves of various gestational ages, cells from tissue taken during the...
متن کاملStructural studies on cross-linked regions of elastin.
A novel use of Edman degradation was made in the study of desmosineand isodesmosine-containing elastolytic peptides of bovine ligamentum nuchae elastin. The elastolysis of elastin produced peptides with the cleavage at or near the NH,-terminals of desmosine and isodesmosine cross-links. Therefore, it became possible to release single chain peptides from the carboxyl groups of these cross-links ...
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Incubation of normal newborn pig aorta with [14C]valine yielded a labeled protein that was isolated and purified with salt-soluble elastin from copper-deficient pig aorta as carrier. The labeled protein is soluble in strong neutral salt solution in the cold. It coacervates reversibly and can be separated into a hydrophobic phase by centrifugation at 32-37”. The protein migrates on polyacrylamid...
متن کاملSelective degradation of accumulated secretory proteins in the endoplasmic reticulum. A possible clearance pathway for abnormal tropoelastin.
The specific pathway of tropoelastin secretion was investigated in fetal calf ligamentum nuchae (FCL) cells using brefeldin A (BFA) to disrupt the secretory pathway. Electron microscopic studies of BFA-treated FCL cells showed ultrastructural changes consistent with the reported effects of BFA on intracellular organelles. When FCL cells were labeled with [3H]leucine in the presence of BFA, radi...
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عنوان ژورنال:
- The Biochemical journal
دوره 141 2 شماره
صفحات -
تاریخ انتشار 1974